(first with the primary antibody and then with the secondary antibody or streptavidin-POD) of up to 4-midi gels, making possible to process up to 56 samples. However, and probably due to the need of working [...] al Ibérica S.L), and EPO-Fc (Prospec), ELISA Kit (Stemcell Technologies), EPO NuPAGE 10% Bis-Tris Midi gels (ThermoFisher), EPO clone AE7A5 (R&D Systems BAM2871), Streptavidin- POD Conjugate (Roche), [...] detection of ERAs by immnunodetection. Its productivity is reduced compared to BlotCyclerrTM, as only 2 midi gels can be processed in the same run. However, it increases cost-effectiveness, as the volume of

(first with the primary antibody and then with the secondary antibody or streptavidin-POD) of up to 4-midi gels, making possible to process up to 56 samples. However, and probably due to the need of working [...] al Ibérica S.L), and EPO-Fc (Prospec), ELISA Kit (Stemcell Technologies), EPO NuPAGE 10% Bis-Tris Midi gels (ThermoFisher), EPO clone AE7A5 (R&D Systems BAM2871), Streptavidin- POD Conjugate (Roche), [...] detection of ERAs by immnunodetection. Its productivity is reduced compared to BlotCyclerrTM, as only 2 midi gels can be processed in the same run. However, it increases cost-effectiveness, as the volume of

were performed according to already described protocol at 150 V on 10% Nupage Novex Bis-Tris 1.0 mm Midi-gels. Semi-dry Western blotting After SDS- and SAR-PAGE, proteins were transferred on Immobilon-P

were performed according to already described protocol at 150 V on 10% Nupage Novex Bis-Tris 1.0 mm Midi-gels. Semi-dry Western blotting After SDS- and SAR-PAGE, proteins were transferred on Immobilon-P